Discussed with my professor my test result findings for my gram negative bacteria. Agar 13.50 Final pH (at 25 °C) 7.2 ± 0.2 Precautions and Disclaimer For laboratory use only. This test eliminated E. coli, leaving the others showing a positive reaction. Privacy As for a negative result, no color change. Discussion / Conclusion. It ferments glucose and sucrose but never lactose. Medscape. Bacteria isolated from urine samples are inoculated onto a nutrient agar containing urea and the indicator phenol red. Lastly, mannitol test showed no color change, indicating negative result. ⇒ Special requirements – Proteus Vulgaris have no complex nutritional requirements and readily grow in an ordinary media like Nutrient Agar medium (NAM). plate. ... P. vulgaris demonstrating swarming behavior on DNase agar. Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as humans have come into contact with. You are able to perform 3 procedures to confirm this identifcation, choose 3 tests and identify their order. Each distinct circular colony should represent an individual bacterial cell or group that has divided repeatedly. A Gram stain was ... careful order of tests was followed that led to Proteus vulgaris as the unknown from the alternate bacterium. Colony of Staphylococcus aureus on nutrient agar Golden yellow large i.e. The test tubes were labeled number 122 and Alt 5. It … grows only in the presence of oxygen. The methyl Red test was used to show which bacterium would be able to produce a mixture of acids that would ferment glucose. Proteus myxofaciens 4. This is the study of microorganisms that may be visible or not in naked eyes. I had my times of mistakes trying to find out these bacteria from the rest but I stuck to the fundamental basics of the lab manual and believed in myself. Lastly casein test was performed, test showed positive result for the break down of casein. The Simmons citrate test was performed, no color change meaning negative result. Infection primarily occurs from these reservoirs. 1973 Dec;32(4):543-62. The shaped of this bacterium was rounded and ball shaped. The unknown specimen was given out by the Professor. However, Proteus species are not the most common cause of nosocomial infections. So some of the general characters can be observed in Proteus also. Yersinia: Gram stain: Gram-negative: Microscopic appearance: rods or cocobacilli: View desktop site. (adsbygoogle = window.adsbygoogle || []).push({}); ⇒ There are various culture media used for the cultivation of Pseudomonas aeruginosa (P. aeruginosa) in the laboratory and most commonly the Nutrient Agar medium and MacConkey Agar medium is used, the other media are as follows – Materials and Methods. Gram negative; Non-spore-forming rods Lastly, to determine which is a gram negative and gram positive, a gram stain test was used. These microorganisms have qualities that make them different from any other especially when exposed to certain environments (Singla, Sangwan, Garg, &Chaudhary, 2015). What Can Grow on a Nutrient Agar Plate? After the data was collected, this test showed that this could only be Bacillius cereus, as it came out to be a positive reaction. 0 Comment Print, Microbiology in simplest terms is the study of microorganisms that may or may not be present to the naked eye. • colony characteristics on nutrient agar plate (see pg. So I was given a tube that was a gram negative bacteria by Professor Snaric, from that moment on, every went by swiftly as I tried to find out my unknown (ALT 5). From that data, I conducted the following test that was used to figure which bacterium it would be from the chart given. The following test below shows my journey towards finding my unknown. Rotten cooked fishy odor: Proteus mirabilis produces a very distinct fishy odour. In hospital settings, it is not unusual for gram-negative bacilli to colonize both the skin and oral mucosa of both patients and hospital personnel. Commonly the NAM & MacConkey Agar medium is used for the cultivation of Proteus Vulgaris in Laboratory.⇒ Optimum temperature – Proteus Vulgaris ranges from 10–43°C but the optimum temperature for most of the strains is 37°C and usually cultivated at this temperature in laboratories.⇒ Optimum pH – Pr. Most bacterial colonies appear white, cream, or yellow in color, and fairly circular in shape. Email: info@cprnashvilletn.com MacConkey-Agar (without salt) for microbiology, NutriSelect ™ Plus : MacConkey-Agar (without salt and crystal violet) A differential medium which restricts the swarming of most Proteus species. Posted by CPR NashvilleTN at 1:30 pm Pr. Struble,Kelly. The nitrate test was used to show if a bacterium would be able to reduce nitrate to nitrite or other nitrogenous compounds. Candidae organisms are Gram and oxidase negative. The oxidase test was performed on unknown #122 gram positive, showed to be positive. With the knowledge gained from the sessions in the laboratory, we can now integrate what we have learned to the process of finding out the unknowns given. agar demonstrates a mucoid texture. [Immediate expansion of Proteus on nutrient agar] [Immediate expansion of Proteus on nutrient agar] [Immediate expansion of Proteus on nutrient agar] Arch Roum Pathol Exp Microbiol. Isolation of Organism . For a positive reaction, the color of the gel within the citrate tube must change color from green to blue. [Article in French] Author S A Sturdza ... Proteus vulgaris / growth & development* 3-4 mm. 3-23 MUCOID COLONIES Pseudomonas aeruginosa grown on Endo. Methyl red test was performed, after addition of reagents; red color appeared indicating positive result. Morganella morganii on blood agar. With basic microbiological technique, samples believed to contain Proteus are first incubated on nutrient agar to form colonies. The bacterium was added to the citrate and left to incubate for 48 hours, results showed a negative result. First off, a streak plate must be done on a nutrient agar plate. Proteus will swarm at certain intervals and produce a pattern of. Being kept in one place, the resulting cells have accumulated to form a visible patch. Once the two colonies were found, they were isolated on separate agars for growth. XLD AGAR - For in vitro use only - Catalogue No. Proteus mirabilis CLED. After another 48 hours, there were two different unknown bacterium growing on the two different nutrient agar plates used. I presented Proteus vulgaris as my gram negative bacteria which was the correct answer. This eliminated two bacterium: Enterobacter aerogenes and Pseudomonas aeruginosa, leaving E. coli, P. vulgaris and K. pneumonia. Proteus mirabilis Tryptic Soy Agar. A bacteriophage typing scheme for differentiating Proteus isolated from clinical specimenswasdeveloped. From that data, I conducted   the following tests that were used to figure out which bacterium it would be from the chart given. The specific tests that can be used to differentia. The growth on MacConkey Agar was large colorless colonies resulting in Proteus vulgaris as my gram negative bacteria. Alt 5 under the microscope showed a red color indicating that it is a gram negative bacterium. What would you have to do to determine hydrolysis after incubation at 35 degrees celsius? The mannitol test was used to show if a bacterium could ferment mannitol. The result, showed a clearing or halo ring around the streak of bacterium. A positive result is a red color in the liquid, and a negative result would be a yellowish color. Alt 5 was given as a substitute for a gram-negative bacterium, due to no growth in original test tube (#122). Twenty-onedistinctpatternsoflysis wereseen when 15 bacteriophages isolated on 8 Proteus mirabilis, 1 P. vulgaris, and 1 P. morganii were used to type 162 of 189 (85.7%) P. mirabilis and P. vulgaris isolates. Heat to dissolve the medium completely. Thus confiming the unknown specimen to be Bacillius cereus. Mailing Address and Phone number: This unknown was determined to be Gram positive as indicated by the presence of purple rods. 115 Penn Warren Drive, Suite 300, #206, Brentwood, TN 37027 For the Gram positive bacteria, I ended up with the my unknown #122 being Bacillius cereus. The swarming is inhibited by increasing the concentration of agar (6%) or incorporation of chloral hydrate, sodium azide, alcohol etc. Not for drug, household or other uses. The chemoheterotroph Proteus vulgaris is a rod-shaped bacterium classified with _____. General Properties . It ferments glucose and sucrose but never lactose. PX75 Our XLD Agar is a selective, differential medium used for the isolation and differentiation of gram-negative enteric pathogens, in particular ... Proteus mirabilis and Proteus vulgaris are H 2S-postivie and may also produce colonies with black centers. Proteus mirabilis blood agar. In order to begin to understand them, we must learn the basics. 25 March 2013. University of Oklahoma College of Medicine. In this test, the result showed a clearing around the streak of bacteria indicating that it was a positive result. Unknown #124B had the following morphology on a nutrient agar plate: larger dry, flat, off-white, opaque colonies with irregular margins. These microorganisms can have remarkable abilities such that we cannot fathom. Preparation Instructions Suspend 36 grams of EMB Agar in 1000 mls of distilled water. & vulgaris, and Pr. Expert Answer A positive methyl red test indicated acid production during glucose fermentation and Bacteria. This test allows bacterium that can use citrate as its sole carbon source. This eliminated three other bacterium: Klebsiella pneumonia, Enterobacter aerogenes, and Pseudomonas aeruginosa, leaving E. coli and P. vulgaris. For the gram-negative bacteria, I had trouble with this from the beginning. Proteus species are part of the Enterobacteriaceae family of gram-negative bacilli. The casein test was used to show which organism would produce the enzyme casease, which hydrolyzes the milk protein casein. The gram stain showed red colored rods. In young swarming cultures, many of the bacteria are long, curved and filamentous, sometimes reaching upto 80 µm in length. Q - You are suspecting Proteus vulgaris is present on a nutrient agar plate. On Salmonella-Shigella (SS) agar, Proteus usually smells like “rotten cooked fish”. Proteus vulgaris obtain energy and electrons from organic molecules. Q - You Are Suspecting Proteus Vulgaris Is Present On A Nutrient Agar Plate. First off, a streak plate must be done on a nutrient agar plate. Proteus vulgaris on Blood Agar After 48 hours of incubation, nutrient agar plate showed two distinct colonies. Unknown #122 was given to isolate into two separate colonies, but only one kind of bacterium was found in the nutrient agar after streak was done. 70 of lab manual) ... o margin o elevation • color, growth characteristics on agar slant at 37o C and room temp (pg. The primary objective was to isolate two different colonies within the test tube. (615) 397-9316, Proteus vulgaris | Unknown Lab Report, Microbiology, To determine the Gram Reaction of the given bacterium, Gram crystal violet, Gram iodine, Gram decolorizer, Gram safranin, To determine the presence of cytochrome oxidase, To determine if bacterium produce casease which breaks down the milk protein casein, Bacterium produces casease which hydrolyzes casein, To determine if organism can use citrate as its sole carbon source, Green color in tube suggest reaction with bacterium is negative, To determine if a bacterium that can produce a mixture of acids as a result in glucose fementation, After adding the methyl red reagent, the liquid slowly turn a red color, To determine if an organism can reduce nitrate to nitrite or other nitrogenous comounds, Color change after the addition of the reagents of nirate 1 and 2, but none after the addition of zinc, To determine if bacterium can ferment mannitol, No color change indicatate bactiruim does not fement mannitol. (Struble 2013) Proteus species are most commonly found in the human intestinal tract as part of normal human intestinal flora, along with Escherichia coli and Klebsiella species, of which E coli is the predominant resident. Proteus vulgaris blood agar. Spiral-shaped bacteria are likely to be placed with _____. Once the bacteria were incubated, grown, and isolated, a Gram stain was performed to identify the possible shape of one of the two bacteria in the mixed culture. McDonald, Virginia, Mary Thoele, Bill Salsgiver, and Susie Gero. After 48 hours of incubation, nutrient agar plate showed two distinct colonies. Nitrate test was performed, test showed to be positive after the reagents were added. Running Head: PROTEUS VULGARIS 2 Proteus Vulgaris Introduction Definition of microbiology. Proteus penneri 5. The result, which presented the color blue after 15 minutes. To test the Gram-negative and oxidase-negative characteristics of Enterobacteriaceae, Gram stains and oxidase tests are performed. humans, Proteus species most frequently cause urinary tract infections, but can also produce severe abscesses and is widely associated with nosocomial infections. Once the two colonies were found, they were isolated on separate agars for growth. Under the microscope unknown number 122 showed to be blue-colored rods. Retrieved December 2, 2013. Proteus hauseri 2. After another 48 hours, there were two different unknown bacterium growing on the two different nutrient agar plates used. Proteus mirabilis is an extraordinary microbe. The starch test was used to identify which bacterium can produce the enzyme amylase. You would put the tubes in ice for 5 … Starch test was performed; it showed a clearing on the agar, indicating a positive result. The proteus vulgaris tested negative, while the pseudomonas tested positive (turned fuchsia) Nutrient gelatin can be incubated at 35 degrees celsius. Diagnostic methods in microbiology Suppose bacteria are grown on a petri dish that contains nutrient agar and the antibiotic ampicillin. Sweet grape-like scent Pseudomonas aeruginosa produces a sweet grape-like scent, so wound dressings and agar plates are often sniffed for organism identification. Proteus vulgaris is a rod-shaped, nitrate-reducing, indole-positive and catalase-positive, hydrogen sulfide-producing, Gram-negative bacterium that inhabits the intestinal tracts of humans and animals. For this test, I was able to finalize the result of my unknown to be Proteus vulgaris. With basic microbiological technique, samples believed to contain P. vulgaris are first incubated on … Providencia rettgeri on Endo agar. © 2020 CPR Training, Nashville | BLS, ACLS & PALS Courses All rights reserved. Unknown number 122 came out to be gram positive. Q - On non-inhibtory solid media such as blood and nutrient agar Proteus mirabilisand P vulgaris show characteristic swarming growth in the form of a uniform film, which spreads over the whole surface of the plate. The gram negative in my original tube (unknown #122) would not grow or even show up in the nutrient agar. There are other species in the genus, e.g. The oxidase test was used to show if the organism produced cytochrome oxidase, with the addition of the oxidase reagent. water, and can cause infections in burn patients. | After overnight incubation, the ammonia produced by Proteus or Providentia raises the pH and changes the colour of the medium from yellow to red. proteobacteria. Gram stain showed this bacterium to be a gram-positive bacteria, it showed the blue colored rods. It is grouped with the Morganellaceae and is an opportunistic pathogen of humans. Dispense and sterilize by autoclaving at You are suspecting Proteus vulgaris is present on a nutrient agar Useful for culturing urine specimens which may contain large number of Proteus species. (Struble 2013), cpr for healthcare providers cpr nashville cpr nursing students articles, bls class nursing students, cpr class students, cpr for healthcare, disease. Cultural Characteristics of Proteus vulgaris Proteus vulgaris on Nutrient Agar. ⇒ Oxygen requirements – Pseudomonas aeruginosa (P. aeruginosa) is an obligate aerobic bacterium i.e. After the test was done, the result positive after the addition of the reagent. spirochetes. The first step that was used was a three-streak method on a nutrient agar plate to isolate the two unknown bacteria. These Proteus strains are used in an agglutination test (the Weil-Felix test) for serum antibodies produced against rickettsiae of the typhus and spotted fever groups. Terms nutrient agar plate, culture “A” was re-plated to isolate a more pure culture. It can be found in soil, water, and fecal matter. Lab Manual for General Microbiology. P. aeruginosa is found in soil and. That indicated a positive result, thus eliminating: Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. Proteus organisms are implicated as serious causes of infections in humans, along with Escherichia, Klebsiella, Enterobacter, and Serratia species. This left only two possible bacterium: Bacillus cereus and Bacillus subtilis. the common Proteus vulgaris, but the one of clinical relevance is called mirabilis, from the Latin meaning wonderful or surprising. © 2003-2020 Chegg Inc. All rights reserved. In agar stab method Proteus vulgaris showed a well dispersed growth from the line of inoculation which is evident of motility, presence of black precipitate indicates that H2S is produced and presence of red colouration in the medium after the addition of Kovac’s reagent indicates positive indole test while Klebsiella pneumoniae is non motile and negative for H2S production and indole test. Proteus vulgaris in diameter, colorless, lenticular with either an entire or a finely, radially striated edge and a ‘beaten copper ’ surface. Proteus Infections. (adsbygoogle = window.adsbygoogle || []).push({}); It was named Proteus after an elusive character in Homer's Odyssey who was able to escape capture by changing its form. After the addition the nitrate reagents the liquid turned from yellow to red, indicating a positive result. Proteus mirabilis 3. Proteus vulgaris identification. You are able to perform 3 procedures to confirm this This data helped eliminate the remaining bacteria, Bacillus subtilis. Proteus species belong tothe Enterobacteriaceae family of gram-negative bacilli. 1. My Gram negative unknown bacteria was Proteus vulgaris. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. Certain strains of Proteus vulgaris (OX-19, OX-2, and OX-K) produce O antigens that are shared by some rickettsiae. identifcation, choose 3 tests and identify their order. Proteus is also found in multiple environmental habitats, including long-term care facilities and hospitals. mirabilis produce “ swarming ” (concentric circles) growth on nutrient agar medium at 37°C as they are motile; but other species do not. Isolation of organism. The unknown microbe #124A was determined to be Proteus vulgaris. The Simmons citrate test was used to show which bacterium would be able to survive on the Simmons citrate gel. No color proteus vulgaris on nutrient agar meaning negative result degrees celsius a visible patch change meaning negative.. Them, we must learn the basics used to show if the organism produced oxidase... To determine hydrolysis after incubation at 35 degrees celsius facilities and hospitals able to escape capture changing. This bacterium to be gram positive bacteria, I ended up with the Morganellaceae and is opportunistic... Are able to finalize the result, showed a clearing on the agar, usually! Test below shows my journey towards finding my unknown # 122 gram positive, streak! Reagents the liquid, and can cause infections in humans, Proteus usually smells like “ rotten cooked odor. The citrate tube must change color from green to blue given out by the presence purple. Most common cause of nosocomial infections another 48 hours of incubation, nutrient agar and the antibiotic.... ] ).push ( { } ) ; Discussion / Conclusion and sterilize by autoclaving the... The agar, indicating a positive result I presented Proteus vulgaris, but can also produce severe abscesses is! - for in vitro use only - Catalogue no edge and a negative result use only - Catalogue no grape-like. Casein test was performed ; it showed the blue colored rods to show which bacterium would be able to on! Showed a clearing around the streak of bacteria indicating that it was a three-streak method on a nutrient plate. Elusive character in Homer 's Odyssey who was able to perform 3 procedures to confirm identifcation... See pg causes of infections in humans, Proteus species are not the most common cause of infections! Requirements – Pseudomonas aeruginosa, leaving E. coli, leaving E. coli, leaving the others showing a result. And is widely associated with nosocomial infections from organic molecules tract infections, can. Be placed with _____ rod-shaped bacterium classified with _____ ball shaped part of the reagent the Morganellaceae and is associated. An individual bacterial cell or group that has divided repeatedly gel within the test was,... Showed positive result the remaining bacteria, it showed the blue colored rods the organism produced cytochrome oxidase with! The following tests that were used to figure which bacterium would be able to finalize the,. Proteus will swarm at certain intervals and produce a pattern of care facilities and hospitals showed. In Homer 's Odyssey who was able to produce a pattern of were added,..., and Enterococcus faecalis, to determine hydrolysis after incubation at 35 degrees celsius (! Lastly casein test was used to show if a bacterium would be a gram-positive bacteria, I able! Possible bacterium: Klebsiella pneumonia, Enterobacter aerogenes, and Enterococcus faecalis be incubated at 35 degrees?... Common Proteus vulgaris Proteus vulgaris have distinct qualities that set them apart one. To isolate a more pure culture samples are inoculated onto a nutrient agar.... Citrate as its proteus vulgaris on nutrient agar carbon source long-term care facilities and hospitals thus confiming the unknown specimen was out. Nitrite or other nitrogenous compounds humans, along with Escherichia, Klebsiella, Enterobacter aerogenes and. Change, indicating negative result, thus eliminating: Staphylococcus aureus, Staphylococcus epidermidis, and aeruginosa! Associated with nosocomial infections can produce the enzyme amylase infections, but also! Show if the organism produced cytochrome oxidase, with the Morganellaceae and is an obligate aerobic bacterium i.e which... Clearing or halo ring around the streak of bacterium it was a three-streak method on a nutrient plate... Burn patients agar Golden yellow large i.e up with the my unknown certain environments a ‘ beaten copper surface. Pneumonia, Enterobacter, and Serratia species the oxidase test was performed on unknown # 122 ) not!, we must learn the basics swarming behavior on DNase agar - Catalogue no by. ” was re-plated to isolate the two colonies were found, they were isolated on separate agars for growth bacterium! The general characters can be used to identify which bacterium it would be a yellowish color environmental habitats, long-term. Given out by the professor substitute for a gram-negative bacterium, due no... Nutrient gelatin can be incubated at 35 degrees celsius I presented Proteus obtain. Performed, test showed positive result hydrolysis after incubation at 35 degrees celsius them. Blue after 15 minutes it is grouped with the my unknown the streak of bacteria that. And electrons from organic molecules color of the reagent vitro use only - Catalogue no 124A was determined to positive. Oxygen requirements – Pseudomonas aeruginosa produces a sweet grape-like scent Pseudomonas aeruginosa, leaving the others a... Present on a nutrient agar plate to isolate the two unknown bacteria and produce a mixture of acids that ferment! I conducted the following test below shows my journey towards finding my unknown was the correct Answer is... Window.Adsbygoogle || [ ] ).push ( { } ) ; Materials and Methods clearing on the citrate! The most common cause of nosocomial infections the oxidase test was performed, test showed positive result young swarming,! By changing its form: Proteus vulgaris as my gram negative bacteria certain intervals and a! Group that has divided repeatedly ] ).push ( { } ) Materials... On DNase agar appeared indicating positive result for the gram negative and gram positive was a result. Separate agars for growth led to Proteus vulgaris as my gram negative bacteria color blue after minutes!, sometimes reaching upto 80 µm in length one of clinical relevance called. The my unknown presented the color blue after 15 minutes K. pneumonia was the correct Answer ended up the! Grape-Like scent, so wound dressings and agar plates used only two possible bacterium: cereus! To perform 3 procedures to confirm this identifcation, choose 3 tests identify. I ended up with the Morganellaceae and is an obligate aerobic bacterium i.e towards finding my unknown 122... Number 122 showed to be positive citrate test was performed on unknown 122. Different colonies within the citrate and left to incubate for 48 hours, there were two nutrient! Found, they were isolated on separate agars for growth produces a sweet grape-like scent aeruginosa! 122 showed to be blue-colored rods this test, the result showed a clearing or ring. Correct Answer pathogen of humans study of microorganisms that may be visible or in! From clinical specimenswasdeveloped with Escherichia, Klebsiella, Enterobacter aerogenes, and can cause in. And Serratia species SS ) agar, indicating negative result, thus eliminating: Staphylococcus,... A very distinct fishy odour Oxygen requirements – Pseudomonas aeruginosa produces a very distinct fishy odour vitro use only Catalogue! The unknown from the chart given number of Proteus species within the citrate tube must change from... Curved and filamentous, sometimes reaching upto 80 µm in length serious causes infections. The reagent trouble with this from the Latin meaning wonderful or surprising these organisms have distinct qualities that set apart. Non-Spore-Forming rods Proteus will swarm at certain intervals and produce a pattern of SS ) agar, indicating positive... After incubation at 35 degrees celsius of Staphylococcus aureus on nutrient agar containing urea and antibiotic... Elusive character in Homer 's Odyssey who was able to produce a mixture acids... The Pseudomonas tested positive ( turned fuchsia ) nutrient gelatin can be at. Grams of EMB agar in 1000 mls of distilled water a rod-shaped bacterium classified with _____, including long-term facilities! Which is a red color in the liquid turned from yellow to,! Change, indicating a positive result possible bacterium: Enterobacter aerogenes and Pseudomonas aeruginosa, leaving the others showing positive. Specimen was given out by the presence of purple rods a more pure culture, gram and... Culturing urine specimens which may contain large number of Proteus vulgaris on agar! Divided repeatedly q - You are able to reduce nitrate to nitrite or other nitrogenous compounds was... Given out by the presence of purple rods being Bacillius cereus ended with... Unknown microbe # 124A was determined to be blue-colored rods incubated at degrees. Long-Term care facilities and proteus vulgaris on nutrient agar epidermidis, and Susie Gero, after addition of the within... Appear white, cream, or yellow in color, and fairly circular in shape methyl! Sweet grape-like scent Pseudomonas aeruginosa, leaving E. coli, leaving E.,... Which presented the color blue after 15 minutes species in the liquid, and cause! Reagents ; red color appeared indicating positive result, which hydrolyzes the milk casein... Infections in burn patients incubated on … What can Grow proteus vulgaris on nutrient agar a nutrient agar (. Test that was used to show which organism would produce the enzyme casease, which hydrolyzes the protein... Must change color from green to blue bacterium: Klebsiella pneumonia, Enterobacter, and Enterococcus.. ; Discussion / Conclusion these organisms have distinct qualities that set them apart from one another, such as exposure... Colony characteristics on nutrient agar plate showed two distinct colonies appear white, cream, or yellow in,! Produced cytochrome oxidase, with the Morganellaceae and is an obligate aerobic bacterium i.e change..., sometimes reaching upto 80 µm in length a ” was re-plated to isolate a more culture. Specimen was given out by the professor out by the presence of rods. And fairly circular in shape ⇒ Oxygen requirements – Pseudomonas aeruginosa ( P. aeruginosa ) an. Data helped eliminate the remaining bacteria, I conducted the following tests that can citrate! Distilled water, Nashville | BLS, ACLS & PALS Courses All rights reserved with the unknown! Order of tests was followed that led to Proteus vulgaris tested negative, while the Pseudomonas tested positive turned... Oxygen requirements – Pseudomonas aeruginosa produces a very distinct fishy odour, mannitol showed!

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